Its history and future as a model for plant-pathogen interactions. Benthamiana is susceptible to a wide variety of other plant-pathogenic.
Benthamiana can be transformed easily it is also.
Nicotiana benthamiana model plant. Its history and future as a model for plant-pathogen interactions. Nicotiana benthamiana is the most widely used experimental host in plant virology due mainly to the large number of diverse plant viruses that can successfully infect it. Benthamiana is susceptible to a wide variety of other plant-pathogenic.
Notwithstanding up until now N. Benthamianas widespread use as a model plant has remained limited due to its allotetraploid nature and the still incomplete sequence of its 31 Gb genome Bally et al 2018. Functional gene studies in this species mostly rely on RNAi based methodologies virus-induced gene silencing VIGS antisense RNA short hairpin RNA shRNA.
Nicotiana benthamiana is widely used as a model plant to analyse cell biology and to obtain insight into the molecular host-pathogen interaction because it is susceptible to many pathogens. Benthamiana can be transformed easily it is also. Nicotiana benthamiana originates from northern Australia and belongs to the Suaveolentes section.
It is used extensively as a model organism for many types of research including plantpathogen interactions RNA interference and functional genomics. Recent publications that used N. Benthamiana as a model for plantpathogen interactions.
Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease. Vladimir Nekrasov Brian Staskawicz Detlef Weigel Jonathan D G Jones Sophien Kamoun. Nicotiana benthamiana is a commonly used model plant for studying plantpathogen interactions because it is host to numerous plant pathogens and because many research tools are available for this species.
The clustered regularly interspaced short palindromic repeats CRISPR system is one of several powerful tools available for targeted gene editing a crucial strategy for analyzing gene function. Here we demonstrate the use of various CRISPR-associated Cas proteins for gene. Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease.
Vladimir Nekrasov 1 Brian Staskawicz 2 Detlef Weigel 3. Nicotiana benthamiana is a widely used model plant species for the study of fundamental questions in molecular plant-microbe interactions and other areas of plant biology. Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease To the Editor.
Sustainable intensification of crop production. Finally we propose that N. Benthamiana is well situated to become a premier plant cell biology model particularly for the virology community who as a group were the first to recognize the.
Nicotiana benthamiana is used extensively as a platform for transient gene expression and as a model system for studying plant-virus interactions. Nicotiana benthamiana a solanaceous species native to Australia is one of the most commonly used model plant organisms. It was initially used by the virology community because of its hypersusceptibility to plant viruses.
Later this feature was exploited with the development of viral vectors that can express foreign genes and the establishment. Nicotiana benthamiana is the most widely used experimental host in plant virology due mainly to the large number of diverse plant viruses that can successfully infect it. Benthamiana is susceptible to a wide variety of other plant-pathogenic agents such as bacteria oomycetes fungi and so on making this species a cornerstone.
Nicotiana benthamiana has become one of the most com-monly used model plant species for research on molecular plant-microbe interactions as well as other areas of plant science Goodin et al. The species was originally adopted by plant virologists because of its susceptibility to numerous viral pathogens Christie and Crawford 1978. This feature led to its.
Benthamiana is a widely used model for plant-microbe biology and other research applications. It is particularly useful because it is related to tomato and potato and is amenable to virus-induced gene silencing VIGS which facilitates the efficient functional study of plant genes. Nicotiana benthamiana is the most widely used experimental host in plant virology due mainly to the large number of diverse plant viruses that can successfully infect it.
Benthamiana is susceptible to a wide variety of other plant-pathogenic agents such as bacteria oomycetes fungi and so on making this species a cornerstone of host-pathogen research. This assay enables transient gene expression in plant. Leaf infilitration of Nicotiana benthamiana with a solution of the bacterium Agrobacterium tumefaciens.