11
Sep
F480 is considered a marker of choice for the identification of mature tissue macrophages being broadly but variably expressed by this cell type in the liver Kupffer cells skin Langerhans cells bone marrow stroma pancreas thymus spleen red pulp lung and other tissues. F480 is expressed at high levels on the surface of various macrophages.
CD11b a C3b receptor is present on the surface of monocytesmacrophages granulocytes and NK cells.
F4 80 positive cells. F480 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68 overall amino acid identity with human EGF module containing mucin like hormone receptor 1 EMR1. Expression of F480 is heterogeneous and is reported to vary during macrophage maturation and activation. Liver mononuclear cells MNCs from C57BL6 mice were isolated and their phenotypes and functions were analyzed.
The effects of clodronate liposomes and gadolinium chloride GdCl3 on Kupffer cells were also investigated. Approximately 25 of liver MNCs were F480 Kupffer cells. Of these 46 were CD11b-CD68 22 were CD11bCD68- and 6 were.
The F480 molecule was established as a unique marker of murine macrophages when a monoclonal antibody was found to recognize an antigen exclusively expressed by these cells. CLEC4F Is an Inducible C-Type Lectin in F480-Positive Cells and Is Involved in Alpha-Galactosylceramide Presentation in Liver. CLEC4F Is an Inducible C-Type Lectin in F480-Positive Cells and Is Involved in Alpha-Galactosylceramide Presentation in Liver Chih-Ya Yang12 Jiun-Bo Chen1 Ting-Fen Tsai3 Yi-Chen Tsai1 Ching-Yen Tsai4 Pi-Hui Liang5 Tsui- Ling Hsu6 Chung.
Representative images of A M1 macrophages F480 and iNOS and B M2 macrophages F480 and CD206 using colon tissue from n 35 mice. F480 positive cells were visualized using Alexa Fluor 594-conjugated goat anti-rat IgG red. Nuclei were stained with.
Ab6640 staining F480 in mouse bone marrow cells by immunocytochemistry immunofluorescence. Cells were formaldehyde fixed and permeabilized in 02 Triton X-100 prior to blocking in 2 BSA for 30 minutes at 20C. The primary antibody was diluted 1200 and incubated with the sample for 9 hour at 4C.
F480 is expressed at high levels on the surface of various macrophages. Kupffer cells splenic red pulp macrophages microglia gut lamina propria and langerhans cells in the skin. Macrophages of the connective tissue heart kidney reproductive and neuroendocrine systems also express F480 Gordon and Austyn 1981.
Monocytes are F480low and most of the monocyte-derived macrophages are F480low. Eosinophils express F480 as well though less than macrophages. In this situation no matter which clone or.
F480 has generally been considered to be a marker specific for both circulating monocytes and tissueresiding monocytes and macrophages yet its expression is not limited to these cell populations 6 7. Here we have demonstrated that F480 is. F480 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68 overall amino acid identity with human EGF module containing mucin like hormone receptor 1 EMR1.
Expression of F480 is heterogeneous and is reported to vary during macrophage maturation and activation. For tissue specific staining the resident red-pulp macrophages in the spleen are F480CD11b lo but resident macrophages the peritoneal cavity they are F480 hiCD11b hi. M1 macrophages were identified as F480-positiveCD11c-positiveCD206-negative and M2 macrophages were identified as F480-positiveCD11c-negativeCD206-positive.
Data analysis was performed using FlowJo software Tree Star Ashland OR USA 6 mice were enrolled in both the OIR and normal control groups for each time point. In intact molars F480-positive cells with dendritic profiles were abundant throughout the dental pulp especially in the perivascular region of the inner pulp and in the OB layer Fig. Two hours after treatment most F480 cells had longitudinal axes directed toward the treated cusp.
F480 is a representative surface marker of mouse mononuclear phagocytes. It is a stable antigen and is not usually present in other types of leukocytes. CD11b a C3b receptor is present on the surface of monocytesmacrophages granulocytes and NK cells.
F480 is considered a marker of choice for the identification of mature tissue macrophages being broadly but variably expressed by this cell type in the liver Kupffer cells skin Langerhans cells bone marrow stroma pancreas thymus spleen red pulp lung and other tissues. Method for isolating F480 positive cells from acute SPEM models. Wild-type C57BL6 mice n3 per sample 3 biological replicate samples were treated with L635 for 3 days or DMP-777 for 10 days and were sacrificed on the last day of treatment.
The corpus was removed from the stomach and enzymatically digested into a single cell suspension. Cells were gated on CD45-positive cells and expression of CD206 and F480 on these cells were analyzed. Flow cytometry analysis showed that the.