Demonstration of nucleic acid

For labeling with biotin a new method of its administration by chemical modification of nucleic acid was employed. Nagpal S1 Sriramarao P Krishnaswamy PR Metcalfe DD Rao PV.

Real-Time Quantitative Polymerase Chain Reaction for the Demonstration of Lyssavirus Nucleic Acid.

Demonstration of nucleic acid. DISCUSSION Although the staining of nucleic acids with basic dyes is not specific a fairly selective demonstration of these substances can be obtained with the methyl greenthionine stain if used under controlled conditions of fixation and staining. As shown previously proteins do not stain. Only nucleic acids and certain sulfated or acidic polysaccharides take up methyl green or thionine.

A methyl green-thionine stain was developed which gives a fairly selective staining of nucleic acids and a sharp differential coloring of chromatin nucleoli and basophil cytoplasm. Ribonucleic acid stains red metachromatically. Proteins and mucins do not stain.

Only the polysaccharides of mast cell granules and cartilage stain also metachromatically. Demonstration of cytomegalovirus nucleic acids in the coronary arteries of transplanted hearts. Hutchins Department of Pathology.

Demonstration of cytomegalovirus nucleic acids in the coronary arteries of transplanted hearts. Wu TC1 Hruban RH Ambinder RF Pizzorno M Cameron DE Baumgartner WA Reitz BA Hayward GS Hutchins GM. 1Department of Pathology Johns Hopkins Medical Institutions Baltimore Maryland.

Nucleus and ribonucleic acid RNA which is located in the cytoplasm of cells mainly in the ribosomes. Both DNA and RNA molecules consist of alternate sugar and phosphate groups with a nitrogenous base being attached to each sugar group. The sugar in DNA is deoxyribose and in RNA it is ribose.

The demonstration of nucleic acid depends upon either the reaction of dyes with the. Demonstration of IgE antibodies to nucleic acid antigens in patients with SLE. Nagpal S1 Sriramarao P Krishnaswamy PR Metcalfe DD Rao PV.

1Department of Biochemistry Indian Institute of Science Bangalore India. Electron microscopical demonstration of nucleic acids in virus-like particles in the skeletal muscle of a traffic accident victim. Acta Neuropathol 47 5559 1979.

DNA-polymerase catalyzes stepwise the incorporation of the nucleotides into the newly synthesized nucleic acid strand. As a result of the incorporation a pyrophosphate PPi molecule is released and subsequently converted to ATP by ATP-sulfurylase. A light signal is produced in the Luciferase reaction during which a luciferin molecule is oxidized.

At the end of each reaction cycle the. Demonstration of borna disease virus nucleic acid in a patient with chronic fatigue syndrome. Nowotny N Kolodziejek J.

Comment on J Infect Dis. 10823802 PubMed - indexed for MEDLINE Publication Types. Borna disease virusisolation purification.

We provide detailed descriptions of methods and materials for successful demonstration of rabies virus RABV nucleic acid by in-situ PCR. The essential components of this topic include sample preparation including care during pre-treatment in-situ reverse transcription RT PCR amplification and detection of target genes. The parameters pertaining to critical steps in the procedure are discussed.

For labeling with biotin a new method of its administration by chemical modification of nucleic acid was employed. In homologous DNADNA hybridization the sensitivity of determinations was less than 1 pg in the biotin-treatment of the probe and 125 pg in its radioactive labeling. Hybridization of DNA probe with cytoplasmic RNA isolated from influenza A virus-infected strains AUSSR9077 and ATexas77.

These complements were chimeras of locked nucleic acids LNAs and regular DNA. Their sequences are listed in Table 2. LNAs are a type of modified nucleic acid.

When hybridized to the same complements LNADNA chimeras have higher melting temperatures than do their regular DNA counterparts of the same sequence. Here such LNADNA chimeras were used to block the polymerase extension reaction. Previously peptide nucleic acids.

In the late 19th century scientists microscopically observed the association of proteins with DNA strands. Since then researchers have used a variety of in vitro and in vivo assays to demonstrate that proteins interact with DNA and RNA to influence the structure and function of the corresponding nucleic acid. Formalin-fixed sections of rat intestine were treated in a solution of 05 periodic acid in 50 aqueous phosphoric acid for the concurrent demonstration of 12-glycols and DNA.

The effects of varying the concentration of reagents time of exposure and temperature on the results showed that at 28C good reactions were obtained in 1015 min when the concentration of the phosphoric was between 40 and 60 and that of the periodic acid. Real-Time Quantitative Polymerase Chain Reaction for the Demonstration of Lyssavirus Nucleic Acid. In comparison with conventional polymerase chain reaction PCR assays real-time PCRs are closed-tube systems thus reducing the possibility of amplicon or cross-contamination that may lead to false positive results.

A generally smaller fragment size causes shorter time for the PCR and. A novel promising strategy for cancer diagnosis and therapy is the use of a pretargeting approach. For this purpose the non-natural DNARNA analogues Peptide Nucleic Acids PNAs are ideal candidates as in vivo recognition units due to their high metabolic stability and lack of unspecific accumulation.

In the pretargeting approach an unlabeled highly specific antibody-PNA conjugate has sufficient time to.