Ck7 positive ck20 negative tumors

Less than 5 525 2650 5175 and more than 75. The percentage of positive cells was recorded as follows.

Figure 1 Using Presence And Absence Of Ck7 And Ck20 To Narrow The Field Of Possible Primary Sites In Cup Patients Technology Assessment On Genetic Testing Or Molecular Pathology Testing Of from www.ncbi.nlm.nih.gov

The sensitivity specificity and positive and negative predictive values of the CK7-CK20 phenotype and of CDX2 expression were analyzed.

Ck7 positive ck20 negative tumors. The sensitivity specificity and positive and negative predictive values of the CK7-CK20 phenotype and of CDX2 expression were analyzed. The CK7-CK20 immunophenotype was expressed by 75 of 118 64 colorectal and 3 of 59 5 gastric tumors and was not observed in any pancreatic adenocarcinomas. CC were positive for CK7 and CK20 in 96 and 70 respectively whatever the architecture and differentiation of the tumor.

The labeling index LI of CK7 in CC was always high whereas it was low or moderate for CK20. CK20-positive phenotype was significantly more frequent in nonperipheral than in peripheral CC 82 vs 47. CRM were all positive for CK20 with a high LI and mostly negative.

Immunohistochemical studies show the cells to be positive for CK7 CAM52 and HER2 while negative for SOX10 p63 CK56 CK20 and adipophilin. The morphologic and immunophenotypic findings are consistent with primary Paget disease of the vulva. Clinical correlation is recommended to exclude metastasis from an extracutaneous site.

Subsequent analysis indicated that a more informative diagnostic segregation could be achieved with a biphasic grading system 50 staining positive. 50 or less negative. Lower intestinal tumors were CK7- and usually CK20 while upper gastrointestinal tumors including those of pancreatobiliary origin were mostly CK7 and CK20-.

Serous papillary ovarian tumors were all CK7 and CK20-. Positive immunostaining for CK7 and CK20 was identified in the cytoplasm cell membrane or both tumor cell components. The immunostaining result was assessed semiquantitatively.

The percentage of positive cells was recorded as follows. Less than 5 525 2650 5175 and more than 75. Cases that stained less than 5 were considered negative and all others as positive.

In accordance with the criteria of Ormsby et al 17 a tumor was considered to be positive for CK7 or CK20 if 50 or more of the tumor cells showed a predominantly intense cytoplasmic. CK7 was expressed in 10 63 of 16 pulmonary carcinoid tumors and only 5 11 of 46 GI carcinoid tumors P. Pancreatic endocrine tumors showed CK7 positivity in 6 50 of 12 cases which was similar to the findings in lung carcinoids and significantly higher than in GI carcinoids P.

On the other hand only six of 84 cases 71 of malignant salivary gland tumors were positive for CK20 including two of 26 cases 77 of mucoepidermoid carcinomas including one. Further information may be given by applying CK7 and CK20 but this will depend on the likely site from which the tumour may have metastasised. If this is breast CK7 and CK20 will be uninformative since both sites are CK7 positive and CK20 negative.

If the probable primary site is colorectum the conclusion is likely to be categorical. Ten patients with CK7-positive and CK20-negative were classified into an unclassified profile. Three patients with CK7-positive and CK20-negative were classified into the digestive n 1 and urothelial n 2 profiles.

One patient with both CK7 and CK20-negative was classified into the unclassified profile. Cytokeratin 7 was positively expressed in primary tumors of lung as well as metastatic tumors from the thyroid and breast. Cytokeratin 20 was negative in all primary lung tumors while positive in metastatic carcinomas from the colon.

Combination of CK7 and CK20 is vastly used to determine the primary site in a metastatic tumor of unknown originThe patte. Watch the full video on IGTV. Combination of CK7 and CK20 is vastly.

Most primary ovarian mucinous adenocarcinomas are immunoreactive for both cytokeratins whereas colonic adenocarcinomas have a predominant CK7-negative CK20-positive immunophenotype. Nonmucinous primary endometrioid and serous adenocarcinomas of the ovary are CK7 positive and CK20 negative. CDX2 is more commonly positive in metastatic gastrointestinal malignancies than in.

Although the large majority 20 of 23. 87 of metastases from the pancreas were CK7 there was a considerable variation in the CK20 staining of these metastases. Five of 23 22 were diffusely positive 4 of 23 17 were focally positive and 14 of 23 61 were negative.

Only 4 of 17 24 of the gallbladder tumors were CK20 half of them only focally but all of them showed CK7. The anatomic site of the tumor in 20 of the 71 cases of CK7 positive CRC and 16 of the 107 cases of CK7 negative CRC was the ascending colon. The difference in the number of cases where the tumor occurred in the ascending colon between CK7 positive and negative CRCs was statistically significant χ 2 4620 P 0032.

The usefulness of CK7 and CK20 in determining the primary site of a carcinoma has been documented for several clinical settings including lung versus colon uterus versus colon and metastatic carcinoma presenting in the liver. 5 - 11 The unique CK profiles of carcinomas from various primary sites make these powerful tools and our results show that CK7 and CK20 coexpression is a useful test to. The expression of CK20 and CK7 in malignant tissue was significantly different in malignant and normal tissues Table 2.

A greater proportion of colorectal cancers was positive for CK20 both CK20CK7 and CK20CK7. Normal tissue was more likely to be negative for CK7 both CK20CK7 and CK20CK7 P.