Cellulose congo red agar composition

The plates were incubated at 37ofor 5 days. We found that growth in Congo red enabled more facile extraction of the ECM from UTI89 biofilms and facilitates isolation of cellulose from the curli mutant UTI89ΔcsgA.

Control of Red Pepper Anthracnose Using Bacillus subtilis YGB36 a.

Cellulose congo red agar composition. The cellulose Congo-Red agar media with the following composition. KH2PO4 05g MgSO4 025g cellulose 2g agar 15g Congo-Red 02g and gelatin 2g. Distilled water 1L and at pH 6872.

PCGrowth on Cellulose medium was confirmed with zones of clearing on Congo red cellulose agar mediumPratima et al2012. Cellulose agar medium composition. KH R2 R PO R 4 R 05g MgSO4 025g Cellulose 20g Agar 15g Gelatin 2g Distilled water 1000ml 1 Litre.

Composition of Cellulose Congo red agar medium. KH R2 R PO R 4 R 05g MgSO R 4 R. Plated on Cellulose Congo red agar Hendricks and Doyle 1995 containing the following composition.

2g Carboxymethyl cellulose 025g MgSO47H2O 05g K2HPO4 2g agar 2g gelatin 02g Congo red in 100ml at pH 7. The plates were incubated at 37ofor 5 days. Screening of Cellulase Producing Bacteria The appearance of clear hydrolysis zones.

Screening of cellulose degrading microorganism was performed by using Congo red test. The isolates were grown on CMC Agar pH 70 and incubated at 37 C for 5 days to allow for the secretion of cellulase. The agar medium was flooded with an aqueous solution of Congo red 1 wv for 15 min to visualize the hydrolysis zone.

The Congo red solution was then poured off and the. Red agar media with the following composition. KH 2 PO 4 05 g MgSO 4 025 g cellulose 2 g agar 15 g Congo-Red 02 g and gelatin 2 g.

Distilled water 1 L and at pH 68. Congo Red is the sodium salt of benzidinediazo-bis-1-naphthylamine-4-sulfonic acid. A diazo dye that is red in alkaline solution and blue in acid solution and used especially as an indicator and as a biological stain.

NCI Thesaurus NCIt Congo Red is an indicator dye that is blue-violet at pH 30 and red. Cellulase CMC agar carboxyl methyl cellulose 5 g MgSO 4 2 g CaCl 2 05 g KCl 1 g FeSO 4 0001 g agar 20 g Sazci 1986. Control of Red Pepper Anthracnose Using Bacillus subtilis YGB36 a.

Other components include gelatin 20 g. And agar 15 g. 1Ldistilled water was added and the pH was maintained at 6872.

For the determination of the isolates capacity to degrade cellulose cellulose CongoRed agar media was used which was enriched with KH. Congo red is an organic compound the sodium salt of 33-11-biphenyl-44-diylbis4-aminonaphthalene-1-sulfonic acidIt is an azo dyeCongo red is water-soluble yielding a red colloidal solution. Its solubility is greater in organic solvents.

However the use of Congo red has long been abandoned primarily because of its carcinogenic properties. A simple inexpensive and sensitive cellulase assay which allows simultaneous enzyme quantitation for many samples has been developed. Radial diffusion of enzyme into substrate incorporated in an agar gel produces zones of hydrolysis which can be visualized by staining undegraded substrate with Congo red.

Degrade carboxymethyl-cellulose CMC based on the decolorization of CMC-basal agar medium using Congo red as a color indicator. They showed the cellulase hydrolysis capacity ranged from 156 to 414. All isolates were Gram positive rod-shaped bacteria and belonged to.

Degrade cellulose from pineapple wastes was investigated by assessing cellulose decomposition by the method of cellulose Congo red agar media hydrolysis capacity HC-value. Of the 8 isolates screened for their fermentation ability 6 showed enhanced performance and. Congo Red 4B Cosmos Red Cotton Red B Cotton Red C Direct Red 28 Direct Red R Direct Red Y.

Empirical Formula Hill Notation. C 32 H 22 N 6 Na 2 O 6 S 2. Congo red is a hallmark amyloid-binding dye and binds to curli yet also binds to cellulose.

We found that growth in Congo red enabled more facile extraction of the ECM from UTI89 biofilms and facilitates isolation of cellulose from the curli mutant UTI89ΔcsgA. Cellulose Congo Red Agar. 0188 ashed acid washed cellulose powder.

002 Congo red 05 Noble Agar 02 gelatin 10volvol sterile soil extract Soil extract prepared as follows105 g of air-dried sieved soil and 660 ml of deionized water are placed in a 1 litre bottle and autoclaved once at 15 psi for 15 minutes then again after 24 hours. Congo red uptake by Rhizobium colonies from yeast extract-mannitol-mineral salts-Congo red-agar plates was related with the cellulose content in the cell capsule of the bacteria. The enzymatic screening for cellulases was based on a carboxymethyl cellulose CMC plate assay whereby the agar medium was mixed with Congo Red CR dye.

The composition of the medium was 1 CMC 15 agar 067 YNB without amino acids and 001 Congo Red. Ten fungal isolates obtained were cultured on CMC-CR agar and incubated at 30C for 72-96 h.